Description:
* Name: Anti-Complement C3 (alpha chain) Antibody
* Description: Mouse monoclonal (6B1) antibody to Complement C3 (alpha chain).
* Applications: WB, ELISA
* Recommended Dilutions: WB: 1:5,000-1:10,000.
* Reactivity: Human
* Immunogen: Recombinant human C3 N-terminal anaphylatoxin construct, amino acids 668-741 of less thana href=" target="_blank" rel="nofollow"greater thanNP_000055.2less than/agreater than.
* Host: Mouse
* Clonality: Monoclonal
* Clone ID: 6B1
* Isotype: IgM
* Conjugate: Unconjugated
* Purification: Protein L purified.
* Concentration: 1 mg/ml
* Product Form: Liquid
* Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Figure 1:
Western blot analysis of Anti-α Subunit Of Complement Component 3 Antibody on human serum samples. Blot of 0.1 μg purified human C3 protein (Lane 1), 10 μg normal human serum proteins (Lane 2) was probed with Anti-α Subunit Of Complement Component 3 Antibody (1:5,000). The Anti-α Subunit Of Complement Component 3 Antibody binds strongly and cleanly to a band at about 115 kDa which represents the intact α subunit of C3 and various proteolytic bands at approximately 68 and 48 kDa. Bands at 190 kDa and above are likely the pro-C3 and its glycosylated form.
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